Details

Autor(en) / Beteiligte

• Delacher, Michael
• Simon, Malte
• Sanderink, Lieke
• Hotz-Wagenblatt, Agnes
• Wuttke, Marina
• Schambeck, Kathrin
• Schmidleithner, Lisa
• Bittner, Sebastian
• Pant, Asmita
• Ritter, Uwe
• Hehlgans, Thomas
• Riegel, Dania
• Schneider, Verena
• Groeber-Becker, Florian Kai
• Eigenberger, Andreas
• Gebhard, Claudia
• Strieder, Nicholas
• Fischer, Alexander
• Rehli, Michael
• Hoffmann, Petra
• Edinger, Matthias
• Strowig, Till
• Huehn, Jochen
• Schmidl, Christian
• Werner, Jens M.
• Prantl, Lukas
• Brors, Benedikt
• Imbusch, Charles D.
• Feuerer, Markus

Titel

Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells

Ist Teil von

• Immunity (Cambridge, Mass.), 2021-04, Vol.54 (4), p.702-720.e17

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Murine regulatory T (Treg) cells in tissues promote tissue homeostasis and regeneration. We sought to identify features that characterize human Treg cells with these functions in healthy tissues. Single-cell chromatin accessibility profiles of murine and human tissue Treg cells defined a conserved, microbiota-independent tissue-repair Treg signature with a prevailing footprint of the transcription factor BATF. This signature, combined with gene expression profiling and TCR fate mapping, identified a population of tissue-like Treg cells in human peripheral blood that expressed BATF, chemokine receptor CCR8 and HLA-DR. Human BATF+CCR8+ Treg cells from normal skin and adipose tissue shared features with nonlymphoid T follicular helper-like (Tfh-like) cells, and induction of a Tfh-like differentiation program in naive human Treg cells partially recapitulated tissue Treg regenerative characteristics, including wound healing potential. Human BATF+CCR8+ Treg cells from healthy tissue share features with tumor-resident Treg cells, highlighting the importance of understanding the context-specific functions of these cells. [Display omitted] •Comparison of chromatin accessibility between murine und human tissue Treg cells•TCR tracking revealed clonal relationship between tissue and blood BATF+CCR8+ Tregs•Treg cells from healthy tissues were similar to CCR8+ Treg cells from tumors•Tfh-like differentiation program induces tissue Treg cell repair characteristics Delacher et al. identify a conserved transcriptional and epigenetic signature that defines tissue regulatory T (Treg) cells in mice and humans. BATF+CCR8+ Treg cells from healthy human tissue share features with Treg cells found in tumors, suggesting that characteristics associated with tumor-residency may rather reflect the tissue repair functions of these cells.