International journal of biological sciences, 2021-01, Vol.17 (4), p.1026-1040
2021
Details
- Autor(en) / Beteiligte
- Titel
- Generation of VEGF knock-in Cashmere goat via the CRISPR/Cas9 system
- Ist Teil von
- International journal of biological sciences, 2021-01, Vol.17 (4), p.1026-1040
- Ort / Verlag
- Australia: Ivyspring International Publisher Pty Ltd
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Electronic Journals Library - Freely accessible e-journals
- Beschreibungen/Notizen
- Cashmere is a rare and specialised animal fibre, which grows on the outer skin of goats. Owing its low yield and soft, light, and warm properties, it has a high economic value. Here, we attempted to improve existing cashmere goat breeds by simultaneously increasing their fibre length and cashmere yield. We attempted this by knocking in the vascular endothelial growth factor ( ) at the fibroblast growth factor 5( ) site using a gene editing technology and then studying its hair growth-promoting mechanisms. We show that a combination of RS-1 and NU7441 significantly improve the efficiency of CRISPR/Cas9-mediated, homologous-directed repair without affecting the embryo cleavage rate or the percentages of embryos at different stages. In addition, we obtained a cashmere goat, which integrated the gene at the site, and the cashmere yield and fibre length of this gene-edited goat were improved. Through next-generation sequencing, we found that the up-regulation of and the down-regulation of affected the cell cycle, proliferation, and vascular tone through the PI3K-AKT signalling pathway and at extracellular matrix-receptor interactions. Owing to this, the gene-edited cashmere goat showed impressive cashmere performance. Overall, in this study, we generated a gene-edited cashmere goat by integrating at the site and provided an animal model for follow-up research on hair growth mechanisms.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1449-2288eISSN: 1449-2288DOI: 10.7150/ijbs.55559Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8040296
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, AKT protein, Animal fibers, Animal Fur - growth & development, Animal models, Animals, Apoptosis, Cashmere, Cell culture, Cell cycle, Cell growth, Cells, Cultured, CRISPR, CRISPR-Cas Systems, Down-regulation, Efficiency, Embryos, Extracellular matrix, Fibroblast growth factor, Fibroblast growth factor 5, Fibroblast Growth Factor 5 - genetics, Fibroblasts, Flow cytometry, Gene Knock-In Techniques, Genetic modification, Goats - genetics, Goats - metabolism, Growth factors, Hair, Homology, Immune system, Laboratory animals, Limited liability companies, Mammals, Next-generation sequencing, Nuclear Transfer Techniques, Plasmids, Prokaryotes, Research Paper, Signal transduction, Skin - metabolism, Textiles, Transcriptome, Vascular endothelial growth factor, Vascular Endothelial Growth Factor A - genetics, Yield