Details

Autor(en) / Beteiligte

• Cullen, Carlie L.
• O'Rourke, Megan
• Beasley, Shannon J.
• Auderset, Loic
• Zhen, Yilan
• Pepper, Renee E.
• Gasperini, Robert
• Young, Kaylene M.

Titel

Kif3a deletion prevents primary cilia assembly on oligodendrocyte progenitor cells, reduces oligodendrogenesis and impairs fine motor function

Ist Teil von

• Glia, 2021-05, Vol.69 (5), p.1184-1203

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Primary cilia are small microtubule‐based organelles capable of transducing signals from growth factor receptors embedded in the cilia membrane. Developmentally, oligodendrocyte progenitor cells (OPCs) express genes associated with primary cilia assembly, disassembly, and signaling, however, the importance of primary cilia for adult myelination has not been explored. We show that OPCs are ciliated in vitro and in vivo, and that they disassemble their primary cilia as they progress through the cell cycle. OPC primary cilia are also disassembled as OPCs differentiate into oligodendrocytes. When kinesin family member 3a (Kif3a), a gene critical for primary cilium assembly, was conditionally deleted from adult OPCs in vivo (Pdgfrα‐CreER™:: Kif3a fl/fl transgenic mice), OPCs failed to assemble primary cilia. Kif3a‐deletion was also associated with reduced OPC proliferation and oligodendrogenesis in the corpus callosum and motor cortex and a progressive impairment of fine motor coordination. Main Points OPCs disassemble primary cilia upon cell cycle re‐entry. Deletion of Kif3a from OPCs prevents primary cilia assembly and reduces OPC proliferation. Deletion of Kif3a from adult OPCs in vivo reduces oligodendrogenesis and impairs motor function.