Details

Autor(en) / Beteiligte

• Chen, Xuemin
• Rostad, Christina A.
• Anderson, Larry J.
• Sun, He-ying
• Lapp, Stacey A.
• Stephens, Kathy
• Hussaini, Laila
• Gibson, Theda
• Rouphael, Nadine
• Anderson, Evan J.

Titel

The development and kinetics of functional antibody-dependent cell-mediated cytotoxicity (ADCC) to SARS-CoV-2 spike protein

Ist Teil von

• Virology (New York, N.Y.), 2021-07, Vol.559, p.1-9

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Since the COVID-19 pandemic, functional non-neutralizing antibody responses to SARS-CoV-2, including antibody-dependent cell-mediated cytotoxicity (ADCC), are poorly understood. We developed an ADCC assay utilizing a stably transfected, dual-reporter target cell line with inducible expression of a SARS-CoV-2 spike protein on the cell surface. Using this assay, we analyzed 61 convalescent serum samples from adults with PCR-confirmed COVID-19 and 15 samples from healthy uninfected controls. We found that 56 of 61 convalescent serum samples induced ADCC killing of SARS-CoV-2 S target cells, whereas none of the 15 healthy controls had detectable ADCC. We then found a modest decline in ADCC titer over a median 3-month follow-up in 21 patients who had serial samples available for analysis. We confirmed that the antibody-dependent target cell lysis was mediated primarily via the NK FcγRIIIa receptor (CD16). This ADCC assay had high sensitivity and specificity for detecting serologic immune responses to SARS-CoV-2. •Inducible EGFP and Luciferase dual reporters target cell line.•Antibody-Dependent Cell-mediated Cytotoxicity (ADCC) to SARS-CoV-2 spike protein.•Cell lysis mediated primarily via the NK FcγRIIIa receptor (CD16).•Functional SARS-CoV-2 spike (S) protein-based ADCC antibody assay.