Details

Autor(en) / Beteiligte

• Lucca, Liliana E
• Axisa, Pierre-Paul
• Lu, Benjamin
• Harnett, Brian
• Jessel, Shlomit
• Zhang, Le
• Raddassi, Khadir
• Zhang, Lin
• Olino, Kelly
• Clune, James
• Singer, Meromit
• Kluger, Harriet M
• Hafler, David A

Titel

Circulating clonally expanded T cells reflect functions of tumor-infiltrating T cells

Ist Teil von

• The Journal of experimental medicine, 2021-04, Vol.218 (4)

Ort / Verlag

United States: Rockefeller University Press

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Understanding the relationship between tumor and peripheral immune environments could allow longitudinal immune monitoring in cancer. Here, we examined whether T cells that share the same TCRαβ and are found in both tumor and blood can be interrogated to gain insight into the ongoing tumor T cell response. Paired transcriptome and TCRαβ repertoire of circulating and tumor-infiltrating T cells were analyzed at the single-cell level from matched tumor and blood from patients with metastatic melanoma. We found that in circulating T cells matching clonally expanded tumor-infiltrating T cells (circulating TILs), gene signatures of effector functions, but not terminal exhaustion, reflect those observed in the tumor. In contrast, features of exhaustion are displayed predominantly by tumor-exclusive T cells. Finally, genes associated with a high degree of blood-tumor TCR sharing were overexpressed in tumor tissue after immunotherapy. These data demonstrate that circulating TILs have unique transcriptional patterns that may have utility for the interrogation of T cell function in cancer immunotherapy.