Details

Autor(en) / Beteiligte

• Rha, Min-Seok
• Jeong, Hye Won
• Ko, Jae-Hoon
• Choi, Seong Jin
• Seo, In-Ho
• Lee, Jeong Seok
• Sa, Moa
• Kim, A Reum
• Joo, Eun-Jeong
• Ahn, Jin Young
• Kim, Jung Ho
• Song, Kyoung-Ho
• Kim, Eu Suk
• Oh, Dong Hyun
• Ahn, Mi Young
• Choi, Hee Kyoung
• Jeon, Ji Hoon
• Choi, Jae-Phil
• Kim, Hong Bin
• Kim, Young Keun
• Park, Su-Hyung
• Choi, Won Suk
• Choi, Jun Yong
• Peck, Kyong Ran
• Shin, Eui-Cheol

Titel

PD-1-Expressing SARS-CoV-2-Specific CD8+ T Cells Are Not Exhausted, but Functional in Patients with COVID-19

Ist Teil von

• Immunity (Cambridge, Mass.), 2021-01, Vol.54 (1), p.44-52.e3

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Memory T cell responses have been demonstrated in COVID-19 convalescents, but ex vivo phenotypes of SARS-CoV-2-specific T cells have been unclear. We detected SARS-CoV-2-specific CD8+ T cells by MHC class I multimer staining and examined their phenotypes and functions in acute and convalescent COVID-19. Multimer+ cells exhibited early differentiated effector-memory phenotypes in the early convalescent phase. The frequency of stem-like memory cells was increased among multimer+ cells in the late convalescent phase. Cytokine secretion assays combined with MHC class I multimer staining revealed that the proportion of interferon-γ (IFN-γ)-producing cells was significantly lower among SARS-CoV-2-specific CD8+ T cells than those specific to influenza A virus. Importantly, the proportion of IFN-γ-producing cells was higher in PD-1+ cells than PD-1− cells among multimer+ cells, indicating that PD-1-expressing, SARS-CoV-2-specific CD8+ T cells are not exhausted, but functional. Our current findings provide information for understanding of SARS-CoV-2-specific CD8+ T cells elicited by infection or vaccination. [Display omitted] •SARS-CoV-2-specific CD8+ T cells are effector memory cells in convalescents•CCR7+CD45RA+ cells are increased among SARS-CoV-2-specific cells in the late phase•SARS-CoV-2-specific CD8+ T cells have fewer IFN-γ+ cells than flu-specific cells•PD-1-expressing SARS-CoV-2-specific CD8+ T cells are not exhausted but functional T cell responses have been demonstrated in COVID-19 patients, but ex vivo phenotypes and functions of SARS-CoV-2-specific T cells remain unclear. Rha et al. examined SARS-CoV-2-specific CD8+ T cells in acute and convalescent COVID-19 patients using MHC class I multimers, finding that PD-1-expressing SARS-CoV-2-specific CD8+ T cells are not exhausted but functional.