Vaccine, 2021-02, Vol.39 (8), p.1241-1247
2021
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- Autor(en) / Beteiligte
- Titel
- Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
- Ist Teil von
- Vaccine, 2021-02, Vol.39 (8), p.1241-1247
- Ort / Verlag
- Netherlands: Elsevier Ltd
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- •RBD linked TT peptide was prepared to high homogeneity at a considerable capacity.•The molecule could stimulate strong humoral and cellular immune response in mice.•It would be the most economical to prevent and eliminate COVID-19 on a global scale. Without approved vaccines and specific treatments, COVID-19 is spreading around the world with above 26 million cases and approximately 864 thousand deaths until now. An efficacious and affordable vaccine is urgently needed. The Val308 – Gly548 of spike protein of SARS-CoV-2 linked with Gln830 – Glu843 of Tetanus toxoid (TT peptide) (designated as S1-4) and without TT peptide (designated as S1-5) were expressed and renatured. The antigenicity and immunogenicity of S1-4 were evaluated by Western Blotting (WB) in vitro and immune responses in mice, respectively. The protective efficiency was measured preliminarily by microneutralization assay (MN50). The soluble S1-4 and S1-5 protein was prepared to high homogeneity and purity. Adjuvanted with Alum, S1-4 protein stimulated a strong antibody response in immunized mice and caused a major Th2-type cellular immunity supplemented with Th1-type immunity. Furthermore, the immunized sera could protect the Vero E6 cells from SARS-CoV-2 infection with neutralizing antibody titer 256. Recombinant SARS-CoV-2 RBD with a built in T helper epitope could stimulate both strong humoral immunity supplemented with cellular immunity in mice, demonstrating that it could be a promising subunit vaccine candidate.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2021.01.044Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7816590
- Format
- –
- Schlagworte
- Animals, Antibodies, Antibodies, Neutralizing - immunology, Antibodies, Viral - immunology, Antibody Formation, Antibody response, Antigenicity, Antigens, Cell-mediated immunity, Chromatography, Coronaviruses, COVID-19, COVID-19 Vaccines - immunology, Disease control, Disease prevention, Enzymes, Epitopes, Epitopes, T-Lymphocyte - immunology, Female, Glycerol, Glycoproteins, Homogeneity, Humans, Humoral immunity, Immune response, Immunity, Immunization, Immunogenicity, Laboratories, Lymphocytes T, Medical research, Mice, Mice, Inbred BALB C, Molecular weight, Neutralization, Peptides, Plasmids, Proteins, RBD, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, Spike Glycoprotein, Coronavirus - genetics, Spike Glycoprotein, Coronavirus - immunology, Spike protein, Subunit vaccine, Tetanus, TT peptide, Vaccines, Viral infections, Viruses, Western blotting