Details

Autor(en) / Beteiligte

• Velusamy, Srinivasan
• Jordak, Katherine
• Kupor, Madeline
• Chochua, Sopio
• McGee, Lesley
• Beall, Bernard
• Burnham, Carey-Ann D
• Burnham, Carey-Ann D.

Titel

Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

Ist Teil von

• Journal of clinical microbiology, 2020-12, Vol.59 (1)

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2020

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 types commonly found in invasive group A (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each real-time PCR assay showed high specificity and accurately identified the respective target type, including subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.