Developmental cell, 2020-12, Vol.55 (6), p.771-783.e5
2020
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- Autor(en) / Beteiligte
- Titel
- Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn’s Disease
- Ist Teil von
- Developmental cell, 2020-12, Vol.55 (6), p.771-783.e5
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2020
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Human gut development requires the orchestrated interaction of differentiating cell types. Here, we generate an in-depth single-cell map of the developing human intestine at 6–10 weeks post-conception. Our analysis reveals the transcriptional profile of cycling epithelial precursor cells; distinct from LGR5-expressing cells. We propose that these cells may contribute to differentiated cell subsets via the generation of LGR5-expressing stem cells and receive signals from surrounding mesenchymal cells. Furthermore, we draw parallels between the transcriptomes of ex vivo tissues and in vitro fetal organoids, revealing the maturation of organoid cultures in a dish. Lastly, we compare scRNA-seq profiles from pediatric Crohn’s disease epithelium alongside matched healthy controls to reveal disease-associated changes in the epithelial composition. Contrasting these with the fetal profiles reveals the re-activation of fetal transcription factors in Crohn’s disease. Our study provides a resource available at www.gutcellatlas.org, and underscores the importance of unraveling fetal development in understanding disease. [Display omitted] •Single-cell RNA-seq map of the developing and pediatric human intestine•Cycling BEX5+ epithelial precursors are distinct from adult LGR5+ stem cells•Human fetal intestinal organoids mature in culture•Fetal transcription factors are reactivated in the Crohn’s disease epithelium Elmentaite and Ross et al. generated a detailed single-cell RNA-seq map of the developing human intestine. Data analyses revealed the presence of an early intestinal epithelial precursor cell and the re-activation of fetal transcription factors in the intestinal epithelium of children diagnosed with Crohn’s disease. The study provides a unique resource accessible to all researchers at www.gutcellatlas.org.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1534-5807eISSN: 1878-1551DOI: 10.1016/j.devcel.2020.11.010Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7762816
- Format
- –
- Schlagworte
- Adolescent, Cells, Cultured, Child, Crohn Disease - genetics, Crohn Disease - metabolism, human fetal gut development, Humans, inflammatory bowel disease, Intestinal Mucosa - embryology, Intestinal Mucosa - metabolism, intestinal organoids, intestinal stem cells, pediatric Crohn's disease, Receptors, G-Protein-Coupled - genetics, Receptors, G-Protein-Coupled - metabolism, Resource, RNA-Seq, Single-Cell Analysis, single-cell RNA sequencing, Transcription Factors - genetics, Transcription Factors - metabolism, Transcriptome, villus formation