Details

Autor(en) / Beteiligte

• Yan, Hongzhu
• Guo, Min
• Zou, Jue
• Xiao, Feng
• Yi, Lina
• He, Ying
• He, Bosheng

Titel

Promotive effect of Talin‐1 protein on gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signaling axis

Ist Teil von

• Journal of clinical laboratory analysis, 2020-12, Vol.34 (12), p.e23555-n/a

Ort / Verlag

United States: John Wiley & Sons, Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective Our research group was aim to explore the molecular mechanism of Talin‐1 protein affecting gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. Methods 12 cases of patients with gastric cancer in this hospital from 2018 to 2019 were collected. Immunohistochemistry assay and Western blotting were used to detect the expression of Talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 protein in gastric cancer tissue. Cell migration and invasion were measured by Transwell. Results The results showed that the expression levels of protein Talin‐1, PXN and MAPK1 in gastric cancer tissues were significantly higher than that in normal tissue. The number of cell adhesion in the model group was significantly lower than that in the normal group. However, the cell adhesion number in ov‐TLN1 was the highest. Transwell results showed that TLN1 could accelerate the migration and invasion abilities of gastric cancer MKN‐45 cells. Moreover, Western blotting showed that protein Talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 in model group all increased compared with normal group. Conclusion It indicated that talin‐1 protein influenced the development of gastric cancer through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. Gastric cancer is one of the most common gastrointestinal malignancies. Adhesion and invasion of gastric cancer cells are related to various signaling pathways in vivo, among which integrin‐activated adhesion kinase‐mediated signaling pathway is an important pathway for tumor invasion and metastasis. Our research group aim was to explore the molecular mechanism of talin‐1 protein affecting gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. The results showed that the expression levels of protein talin‐1, PXN, and MAPK1 in gastric cancer tissues were significantly higher than that in normal tissue. The number of cell adhesion in the model group was significantly lower than that in the normal group. However, the cell adhesion number in ov‐TLN1 was the highest. Transwell showed that TLN1 can accelerate the migration and invasion abilities of gastric cancer MKN‐45 cells. Moreover, Western blotting showed that protein talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 in model group all increased compared with normal group. It indicated that talin‐1 protein influenced the development of gastric cancer through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis.