RNA (Cambridge), 2021-01, Vol.27 (1), p.106-121
2021
Details
- Autor(en) / Beteiligte
- Titel
- PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
- Ist Teil von
- RNA (Cambridge), 2021-01, Vol.27 (1), p.106-121
- Ort / Verlag
- United States: Cold Spring Harbor Laboratory Press
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Telomeric repeat-containing RNA (TERRA) molecules play important roles at telomeres, from heterochromatin regulation to telomerase activity control. In human cells, TERRA is transcribed from subtelomeric promoters located on most chromosome ends and associates with telomeres. The origin of mouse TERRA molecules is, however, unclear, as transcription from the pseudoautosomal PAR locus was recently suggested to account for the vast majority of TERRA in embryonic stem cells (ESC). Here, we confirm the production of TERRA from both the chromosome 18q telomere and the PAR locus in mouse embryonic fibroblasts, ESC, and various mouse cancer and immortalized cell lines, and we identify two novel sources of TERRA on mouse chromosome 2 and X. Using various approaches, we show that PAR-TERRA molecules account for the majority of TERRA transcripts, displaying an increase of two to four orders of magnitude compared to the telomeric 18q transcript. Finally, we present a SILAC-based pull-down screen revealing a large overlap between TERRA-interacting proteins in human and mouse cells, including PRC2 complex subunits, chromatin remodeling factors, DNA replication proteins, Aurora kinases, shelterin complex subunits, Bloom helicase, Coilin, and paraspeckle proteins. Hence, despite originating from distinct genomic regions, mouse and human TERRA are likely to play similar functions in cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1355-8382eISSN: 1469-9001DOI: 10.1261/rna.076281.120Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7749631
- Format
- –
- Schlagworte
- Animals, Aurora Kinases - genetics, Aurora Kinases - metabolism, Cell Line, Tumor, Chromatin remodeling, Chromosome 18, Chromosome 2, Chromosomes, Chromosomes, Mammalian - chemistry, Chromosomes, Mammalian - metabolism, Computational Biology - methods, DNA biosynthesis, DNA helicase, Embryo cells, Embryo fibroblasts, Fibroblasts - cytology, Fibroblasts - metabolism, Gene Expression Regulation, Neoplastic, Gene Regulatory Networks, Granulocyte Precursor Cells - cytology, Granulocyte Precursor Cells - metabolism, HeLa Cells, Heterochromatin, Humans, Mice, Monocytes - cytology, Monocytes - metabolism, Mouse Embryonic Stem Cells - cytology, Mouse Embryonic Stem Cells - metabolism, Neoplasms - genetics, Neoplasms - metabolism, Neoplasms - pathology, Neurons - cytology, Neurons - metabolism, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Proteins, RecQ Helicases - genetics, RecQ Helicases - metabolism, RNA, Messenger - classification, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNA-Binding Proteins - classification, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, Shelterin Complex, Stem cell transplantation, Stem cells, Telomerase, Telomere - chemistry, Telomere - metabolism, Telomere-Binding Proteins - genetics, Telomere-Binding Proteins - metabolism, Telomeres, Transcription, Transcriptome, Tumor cell lines