Details

Autor(en) / Beteiligte

• Jiang, Tian
• Li, Chenyi
• Teng, Yuxi
• Zhang, Ruihua
• Yan, Yajun

Titel

Recent advances in improving metabolic robustness of microbial cell factories

Ist Teil von

• Current opinion in biotechnology, 2020-12, Vol.66, p.69-77

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2020

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• [Display omitted] •Robustness engineering is essential to increase the production performance of microbial cell factories.•Typical strategies for enhancing the microbial host robustness were reviewed and summarized.•The challenges and future perspectives of robustness engineering were discussed. Engineering microbial cell factories has been widely applied to produce compounds spanning from intricate natural products to bulk commodities. In each case, host robustness is essential to ensure the reliable and sustainable production of targeted metabolites. However, it can be negatively affected by metabolic burden, pathway toxicity, and harsh environment, resulting in a decreased titer and productivity. Enhanced robustness enables host to have better production performance under complicated growth circumstances. Here, we review current strategies for boosting host robustness, including metabolic balancing, genetic and phenotype stability enhancement, and tolerance engineering. In addition, we discuss the challenges and future perspectives on microbial host engineering for increased robustness.