Details

Autor(en) / Beteiligte

• Weisel, Nadine M.
• Weisel, Florian J.
• Farber, Donna L.
• Borghesi, Lisa A.
• Shen, Yufeng
• Ma, Wenji
• Luning Prak, Eline T.
• Shlomchik, Mark J.

Titel

Comprehensive analyses of B-cell compartments across the human body reveal novel subsets and a gut-resident memory phenotype

Ist Teil von

• Blood, 2020-12, Vol.136 (24), p.2774-2785

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Although human B cells have been extensively studied, most reports have used peripheral blood as a source. Here, we used a unique tissue resource derived from healthy organ donors to deeply characterize human B-cell compartments across multiple tissues and donors. These datasets revealed that B cells in the blood are not in homeostasis with compartments in other tissues. We found striking donor-to-donor variability in the frequencies and isotype of CD27+ memory B cells (MBCs). A comprehensive antibody-based screen revealed markers of MBC and allowed identification of novel MBC subsets with distinct functions defined according to surface expression of CD69 and CD45RB. We defined a tissue-resident MBC phenotype that was predominant in the gut but absent in blood. RNA-sequencing of MBC subsets from multiple tissues revealed a tissue-resident MBC gene signature as well as gut- and spleen-specific signatures. Overall, these studies provide novel insights into the nature and function of human B-cell compartments across multiple tissues. •Analysis of B lineage cells in tissues of healthy organ donors reveals tissue-specific differences and discordance between blood and tissues.•New memory B-cell subsets were discovered, including 1 subset specific to the gut that has unique surface markers and gene expression. [Display omitted]