Details

Autor(en) / Beteiligte

• Schwitalla, Jan W
• Benndorf, René
• Martin, Karin
• Vollmers, John
• Kaster, Anne-Kristin
• de Beer, Z Wilhelm
• Poulsen, Michael
• Beemelmanns, Christine

Titel

Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2020-11, Vol.70 (11), p.5806-5811

Ort / Verlag

England: Microbiology Society

Erscheinungsjahr

2020

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5 , was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite , showed morphological, physiological and chemotaxonomic properties typical of the genus . Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5 was DSM 42102 (98.87 %). DNA-DNA hybridization experiments between strain RB5 and DSM 42102 resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5 contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H ) and MK-9(H )]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C ) 12-methyltetradecanoic acid (anteiso-C ), 13-methyltetradecanoic acid (iso-C ) and 14-methylpentadecanoic acid (iso-C ). Here, we propose a novel species of the genus with the type strain RB5 (=VKM Ac-2839 =NRRL B65539 ).