Details

Autor(en) / Beteiligte

• Paniagua-Herranz, Lucía
• Menéndez-Méndez, Aida
• Gómez-Villafuertes, Rosa
• Olivos-Oré, Luis A.
• Biscaia, Miguel
• Gualix, Javier
• Pérez-Sen, Raquel
• Delicado, Esmerilda G.
• Artalejo, Antonio R.
• Miras-Portugal, María Teresa
• Ortega, Felipe

Titel

Live Imaging Reveals Cerebellar Neural Stem Cell Dynamics and the Role of VNUT in Lineage Progression

Ist Teil von

• Stem cell reports, 2020-11, Vol.15 (5), p.1080-1094

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Little is known about the intrinsic specification of postnatal cerebellar neural stem cells (NSCs) and to what extent they depend on information from their local niche. Here, we have used an adapted cell preparation of isolated postnatal NSCs and live imaging to demonstrate that cerebellar progenitors maintain their neurogenic nature by displaying hallmarks of NSCs. Furthermore, by using this preparation, all the cell types produced postnatally in the cerebellum, in similar relative proportions to those observed in vivo, can be monitored. The fact that neurogenesis occurs in such organized manner in the absence of signals from the local environment, suggests that cerebellar lineage progression is to an important extent governed by cell-intrinsic or pre-programmed events. Finally, we took advantage of the absence of the niche to assay the influence of the vesicular nucleotide transporter inhibition, which dramatically reduced the number of NSCs in vitro by promoting their progression toward neurogenesis. [Display omitted] •We present a preparation that allows monitoring the behavior of cerebellar NSCs•Isolated NSCs maintain their neurogenic nature in absence of niche factors•The model enables monitoring the three postnatal cerebellar niches simultaneously•VNUT influences the balance between quiescence and activation of cerebellar NSCs In this article, Ortega and colleagues describe a cell preparation that allows, in combination with live imaging, monitoring the behavior of NSCs from the three postnatal cerebellar niches. The authors provide evidence that, despite the absence of niche signals, isolated NSCs maintain their neurogenic nature and suggest a role for VNUT in the decision between quiescence and activation of cerebellar NSCs.