Details

Autor(en) / Beteiligte

• Forte, Dorian
• García-Fernández, María
• Sánchez-Aguilera, Abel
• Stavropoulou, Vaia
• Fielding, Claire
• Martín-Pérez, Daniel
• López, Juan Antonio
• Costa, Ana S.H.
• Tronci, Laura
• Nikitopoulou, Efterpi
• Barber, Michael
• Gallipoli, Paolo
• Marando, Ludovica
• Fernández de Castillejo, Carlos López
• Tzankov, Alexandar
• Dietmann, Sabine
• Cavo, Michele
• Catani, Lucia
• Curti, Antonio
• Vázquez, Jesús
• Frezza, Christian
• Huntly, Brian J.
• Schwaller, Juerg
• Méndez-Ferrer, Simón

Titel

Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy

Ist Teil von

• Cell metabolism, 2020-11, Vol.32 (5), p.829-843.e9

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Like normal hematopoietic stem cells, leukemic stem cells depend on their bone marrow (BM) microenvironment for survival, but the underlying mechanisms remain largely unknown. We have studied the contribution of nestin+ BM mesenchymal stem cells (BMSCs) to MLL-AF9-driven acute myeloid leukemia (AML) development and chemoresistance in vivo. Unlike bulk stroma, nestin+ BMSC numbers are not reduced in AML, but their function changes to support AML cells, at the expense of non-mutated hematopoietic stem cells (HSCs). Nestin+ cell depletion delays leukemogenesis in primary AML mice and selectively decreases AML, but not normal, cells in chimeric mice. Nestin+ BMSCs support survival and chemotherapy relapse of AML through increased oxidative phosphorylation, tricarboxylic acid (TCA) cycle activity, and glutathione (GSH)-mediated antioxidant defense. Therefore, AML cells co-opt energy sources and antioxidant defense mechanisms from BMSCs to survive chemotherapy. [Display omitted] •Nestin+ BMSCs support leukemogenesis and chemoresistance•BMSCs support metabolic requirements of LSCs•BMSCs provide LSCs with essential antioxidant defense from chemotherapy•GSH and GSH peroxidases underlie BMSC-derived antioxidant AML protection Forte et al. reveal that nestin+ bone marrow stromal cells directly contribute to leukemogenesis and chemotherapy resistance in an in vivo model of acute myeloid leukemia. Nestin+ BMSCs support leukemic stem cells through a dual mechanism of increased bioenergetic capacity through OXPHOS and TCA and glutathione-dependent antioxidant defense.