Details

Autor(en) / Beteiligte

• Huang, Qiang
• Cohen, Malkiel A
• Alsina, Fernando C
• Devlin, Garth
• Garrett, Aliesha
• McKey, Jennifer
• Havlik, Patrick
• Rakhilin, Nikolai
• Wang, Ergang
• Xiang, Kun
• Mathews, Parker
• Wang, Lihua
• Bock, Cheryl
• Ruthig, Victor
• Wang, Yi
• Negrete, Marcos
• Wong, Chi Wut
• Murthy, Preetish K L
• Zhang, Shupei
• Daniel, Andrea R
• Kirsch, David G
• Kang, Yubin
• Capel, Blanche
• Asokan, Aravind
• Silver, Debra L
• Jaenisch, Rudolf
• Shen, Xiling

Titel

Intravital imaging of mouse embryos

Ist Teil von

• Science (American Association for the Advancement of Science), 2020-04, Vol.368 (6487), p.181-186

Ort / Verlag

United States: The American Association for the Advancement of Science

Erscheinungsjahr

2020

Link zum Volltext

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.