Details

Autor(en) / Beteiligte

• Mousavi-Nasab, Seyed Dawood
• Sabahi, Farzaneh
• Kaghazian, Hooman
• Paryan, Mahdi
• Mirab Samiee, Siamak
• Ghaderi, Mostafa
• Zali, Fatemeh
• Makvandi, Manoochehr

Titel

A Real-Time RT-PCR Assay for Genotyping of Rotavirus

Ist Teil von

• Iranian biomedical journal, 2020-10, Vol.24 (6), p.399-399

Ort / Verlag

Tehran: Pasteur Institute of Iran

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Background: HRV is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on reverse transcription PCR for rapid, specific and sensitive genotyping of rotaviruses. This study describes an alternative real-time PCR assay for genotyping of rotavirus. Methods: The samples of stools studied in this research have been collected from patients referred to Children's Medical Centers, Tehran, Iran. Rotavirus detection and genotyping were performed using the RT-PCR and semi-nested RT-PCR, respectively. Samples were then genotyped with a new real-time PCR. Results: The real-time PCR was able to genotype all positive samples with a mean Ct of 28.2. Besides, a concordance rate of 100% was detected between real-time PCR and semi-nested RT-PCR. Conclusion: In this study, the genotyping of rotavirus with real-time PCR showed that this method can provide several favorable features, including high sensitivity and specificity, and a wide dynamic range for rotavirus genotyping.