Diabetes (New York, N.Y.), 2020-11, Vol.69 (11), p.2446-2457
2020
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Details
- Autor(en) / Beteiligte
- Titel
- YAP Activation in Renal Proximal Tubule Cells Drives Diabetic Renal Interstitial Fibrogenesis
- Ist Teil von
- Diabetes (New York, N.Y.), 2020-11, Vol.69 (11), p.2446-2457
- Ort / Verlag
- United States: American Diabetes Association
- Erscheinungsjahr
- 2020
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- An increasing number of studies suggest that the renal proximal tubule is a site of injury in diabetic nephropathy (DN), and progressive renal tubulointerstitial fibrosis is an important mediator of progressive kidney dysfunction in DN. In this study, we observed increased expression and activation of YAP (yes-associated protein) in renal proximal tubule epithelial cells (RPTC) in patients with diabetes and in mouse kidneys. Inducible deletion of specifically in RPTC or administration of the YAP inhibitor verteporfin significantly attenuated diabetic tubulointerstitial fibrosis. EGFR-dependent activation of RhoA/Rock and PI3K-Akt signals and their reciprocal interaction were upstream of proximal tubule YAP activation in diabetic kidneys. Production and release of CTGF in culture medium were significantly augmented in human embryonic kidney (HEK)-293 cells transfected with a constitutively active YAP mutant, and the conditioned medium collected from these cells activated and transduced fibroblasts into myofibroblasts. This study demonstrates that proximal tubule YAP-dependent paracrine mechanisms play an important role in diabetic interstitial fibrogenesis; therefore, targeting Hippo signaling may be a therapeutic strategy to prevent the development and progression of diabetic interstitial fibrogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0012-1797eISSN: 1939-327XDOI: 10.2337/db20-0579Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7576565
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, Adaptor Proteins, Signal Transducing - genetics, Adaptor Proteins, Signal Transducing - metabolism, AKT protein, Amides - pharmacology, Animals, Cell culture, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Chromones - pharmacology, Clear cell-type renal cell carcinoma, Complications, Connective tissue growth factor, Diabetes, Diabetes mellitus, Diabetes Mellitus, Experimental - chemically induced, Diabetes Mellitus, Experimental - complications, Diabetes Mellitus, Experimental - metabolism, Diabetic nephropathy, Epidermal growth factor receptors, Epithelial cells, ErbB Receptors - genetics, ErbB Receptors - metabolism, Fibroblasts, Fibrosis, Fibrosis - metabolism, Fibrosis - pathology, Gene Expression Regulation - drug effects, HEK293 Cells, Humans, Kidney diseases, Kidney Diseases - metabolism, Kidney Diseases - pathology, Kidneys, Mice, Mice, Knockout, Morpholines - pharmacology, Nephropathy, Nitric Oxide Synthase Type III - genetics, Nitric Oxide Synthase Type III - metabolism, Paracrine signalling, Pyridines - pharmacology, Rho-associated kinase, rho-Associated Kinases - genetics, rho-Associated Kinases - metabolism, rhoA GTP-Binding Protein - genetics, rhoA GTP-Binding Protein - metabolism, RhoA protein, Signal Transduction - drug effects, Signal Transduction - physiology, Sodium, Transcription Factors - genetics, Transcription Factors - metabolism, Yes-associated protein