Details

Autor(en) / Beteiligte

• Kiran, Uday
• Gokulan, C G
• Kuncha, Santosh Kumar
• Vedagiri, Dhiviya
• Chander, Bingi Thrilok
• Sekhar, Aedula Vinaya
• Dontamala, Suchitra
• Reddy, Arakatla Lohith
• Tallapaka, Karthik Bharadwaj
• Mishra, Rakesh K
• Harshan, Krishnan Harinivas

Titel

Easing diagnosis and pushing the detection limits of SARS-CoV-2

Ist Teil von

• Biology methods and protocols, 2020-01, Vol.5 (1), p.bpaa017-bpaa017

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Abstract Rigorous testing is the way forward to fight the coronavirus disease 2019 pandemic. Here we show that the currently used and most reliable reverse transcription-polymerase chain reaction-based severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) procedure can be further simplified to make it faster, safer, and economical by eliminating the RNA isolation step. The modified method is not only fast and convenient but also at par with the traditional method in terms of accuracy, and therefore can be used for mass screening. Our method takes about half the time and is cheaper by ∼40% compared to the currently used method. We also provide a variant of the new method that increases the efficiency of detection by ∼30% compared to the existing procedure. Taken together, we demonstrate a more effective and reliable method of SARS-CoV-2 detection.