Details

Autor(en) / Beteiligte

• Otto, Nils
• Pleijzier, Markus W.
• Morgan, Isabel C.
• Edmondson-Stait, Amelia J.
• Heinz, Konrad J.
• Stark, Ildiko
• Dempsey, Georgia
• Ito, Masayoshi
• Kapoor, Ishaan
• Hsu, Joseph
• Schlegel, Philipp M.
• Bates, Alexander S.
• Feng, Li
• Costa, Marta
• Ito, Kei
• Bock, Davi D.
• Rubin, Gerald M.
• Jefferis, Gregory S.X.E.
• Waddell, Scott

Titel

Input Connectivity Reveals Additional Heterogeneity of Dopaminergic Reinforcement in Drosophila

Ist Teil von

• Current biology, 2020-08, Vol.30 (16), p.3200-3211.e8

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2020

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Different types of Drosophila dopaminergic neurons (DANs) reinforce memories of unique valence and provide state-dependent motivational control [1]. Prior studies suggest that the compartment architecture of the mushroom body (MB) is the relevant resolution for distinct DAN functions [2, 3]. Here we used a recent electron microscope volume of the fly brain [4] to reconstruct the fine anatomy of individual DANs within three MB compartments. We find the 20 DANs of the γ5 compartment, at least some of which provide reward teaching signals, can be clustered into 5 anatomical subtypes that innervate different regions within γ5. Reconstructing 821 upstream neurons reveals input selectivity, supporting the functional relevance of DAN sub-classification. Only one PAM-γ5 DAN subtype γ5(fb) receives direct recurrent feedback from γ5β′2a mushroom body output neurons (MBONs) and behavioral experiments distinguish a role for these DANs in memory revaluation from those reinforcing sugar memory. Other DAN subtypes receive major, and potentially reinforcing, inputs from putative gustatory interneurons or lateral horn neurons, which can also relay indirect feedback from MBONs. We similarly reconstructed the single aversively reinforcing PPL1-γ1pedc DAN. The γ1pedc DAN inputs mostly differ from those of γ5 DANs and they cluster onto distinct dendritic branches, presumably separating its established roles in aversive reinforcement and appetitive motivation [5, 6]. Tracing also identified neurons that provide broad input to γ5, β′2a, and γ1pedc DANs, suggesting that distributed DAN populations can be coordinately regulated. These connectomic and behavioral analyses therefore reveal further complexity of dopaminergic reinforcement circuits between and within MB compartments. •Nanoscale anatomy reveals additional subtypes of rewarding dopaminergic neurons•Connectomics reveals input specificity to subtypes of dopaminergic neurons•Axon morphology implies dopaminergic neurons provide subcompartment-level function•Unique dopaminergic subtypes serve aversive memory extinction and sugar learning Otto et al. use electron microscope resolution connectomics to describe the structure and organization of neurons providing synaptic input to functionally discrete subtypes of dopaminergic neurons. The nanoscale anatomy reveals further anatomical and functional specialization of dopaminergic neurons, which is confirmed with behavioral experiments.