Details

Autor(en) / Beteiligte

• McNulty, Erin E
• Nalls, Amy V
• Xun, Randy
• Denkers, Nathaniel D
• Hoover, Edward A
• Mathiason, Candace K

Titel

In vitro detection of haematogenous prions in white-tailed deer orally dosed with low concentrations of chronic wasting disease

Ist Teil von

• Journal of general virology, 2020-03, Vol.101 (3), p.347-361

Ort / Verlag

England: Microbiology Society

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Infectivity associated with prion disease has been demonstrated in blood throughout the course of disease, yet the ability to detect blood-borne prions by methods remains challenging. We capitalized on longitudinal pathogenesis studies of chronic wasting disease (CWD) conducted in the native host to examine haematogenous prion load by real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification. Our study demonstrated detection of amyloid seeding activity (prions) in buffy-coat cells harvested from deer orally dosed with low concentrations of CWD positive (+) brain (1 gr and 300 ng) or saliva (300 ng RT-QuIC equivalent). These findings make possible the longitudinal assessment of prion disease and deeper investigation of the role haematogenous prions play in prion pathogenesis.