Details

Autor(en) / Beteiligte

• Sorrentino, Andrea
• Michel, Thomas

Titel

Redox à la carte: Novel chemogenetic models of heart failure

Ist Teil von

• British journal of pharmacology, 2020-07, Vol.177 (14), p.3162-3167

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Many current animal models of heart failure are hampered by intrinsic methodological complexities, while other models yield only a subtle cardiac phenotype even after prolonged in vivo treatments. A new ‘chemogenetic’ animal model of heart failure reproduces a critical characteristic shared by many disease states that lead to heart failure in humans: an increase in redox stress in the heart. This ‘chemogenetic’ approach exploits a recombinant yeast enzyme that can be dynamically and specifically activated in vivo to generate the ROS hydrogen peroxide (H2O2) in cardiac myocytes. Redox stress can be rapidly, selectively and reversibly manipulated by chemogenetic generation of ROS in cardiac myocytes, yielding a new model of dilated cardiomyopathy. Treatment of animals with the angiotensin receptor antagonist valsartan promotes recovery of ventricular function and resolution of adverse cardiac remodelling. This mini‐review discusses in vivo chemogenetic approaches to manipulate and analyse oxidative stress in the heart.