Details

Autor(en) / Beteiligte

• Brazee, Patricia L
• Morales-Nebreda, Luisa
• Magnani, Natalia D
• Garcia, Joe Gn
• Misharin, Alexander V
• Ridge, Karen M
• Budinger, G R Scott
• Iwai, Kazuhiro
• Dada, Laura A
• Sznajder, Jacob I

Titel

Linear ubiquitin assembly complex regulates lung epithelial-driven responses during influenza infection

Ist Teil von

• The Journal of clinical investigation, 2020-03, Vol.130 (3), p.1301-1314

Ort / Verlag

United States: American Society for Clinical Investigation

Erscheinungsjahr

2020

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Influenza A virus (IAV) is among the most common causes of pneumonia-related death worldwide. Pulmonary epithelial cells are the primary target for viral infection and replication and respond by releasing inflammatory mediators that recruit immune cells to mount the host response. Severe lung injury and death during IAV infection result from an exuberant host inflammatory response. The linear ubiquitin assembly complex (LUBAC), composed of SHARPIN, HOIL-1L, and HOIP, is a critical regulator of NF-κB-dependent inflammation. Using mice with lung epithelial-specific deletions of HOIL-1L or HOIP in a model of IAV infection, we provided evidence that, while a reduction in the inflammatory response was beneficial, ablation of the LUBAC-dependent lung epithelial-driven response worsened lung injury and increased mortality. Moreover, we described a mechanism for the upregulation of HOIL-1L in infected and noninfected cells triggered by the activation of type I IFN receptor and mediated by IRF1, which was maladaptive and contributed to hyperinflammation. Thus, we propose that lung epithelial LUBAC acts as a molecular rheostat that could be selectively targeted to modulate the immune response in patients with severe IAV-induced pneumonia.