Details

Autor(en) / Beteiligte

• Stefanovski, Darko
• Shih, Pei-an Betty
• Hammock, Bruce D.
• Watanabe, Richard M.
• Youn, Jang H.

Titel

Assessment of soluble epoxide hydrolase activity in vivo: A metabolomic approach

Ist Teil von

• Prostaglandins & other lipid mediators, 2020-06, Vol.148, p.106410-106410, Article 106410

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Soluble epoxide hydrolase (sEH) converts several FFA epoxides to corresponding diols.•Epoxide-diol ratios correlated strongly with each other, suggesting these ratios all reflect changes in sEH activity.•A novel modeling approach to analyze all epoxide-diol ratios simultaneously, termed SAMI, was developed.•SAMI improves power in detecting changes in sEH activity in animals and humans when compared to individual ratio estimates. Soluble epoxide hydrolase (sEH) converts several FFA epoxides to corresponding diols. As many as 15 FFA epoxide-diol ratios are measured to infer sEH activity from their ratios. Using previous data, we assessed if individual epoxide-diol ratios all behave similarly to reflect changes in sEH activity, and whether analyzing these ratios together increases the power to detect changes in in-vivo sEH activity. We demonstrated that epoxide-diol ratios correlated strongly with each other (P < 0.05), suggesting these ratios all reflect changes in sEH activity. Furthermore, we developed a modeling approach to analyze all epoxide-diol ratios simultaneously to infer global sEH activity, named SAMI (Simultaneous Analysis of Multiple Indices). SAMI improved power in detecting changes in sEH activity in animals and humans when compared to individual ratio estimates. Thus, we introduce a new powerful method to infer sEH activity by combining metabolomic determination and simultaneous analysis of all measurable epoxide-diol pairs.