Microbiology and immunology, 2018-09, Vol.62 (9), p.574-584
2018
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- Autor(en) / Beteiligte
- Titel
- Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus
- Ist Teil von
- Microbiology and immunology, 2018-09, Vol.62 (9), p.574-584
- Ort / Verlag
- Australia: Wiley Subscription Services, Inc
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- ABSTRACT Middle East respiratory syndrome coronavirus (MERS‐CoV) is a single‐stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS‐CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS‐CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS‐CoV. Using a recombinant full‐length S protein, an indirect ELISA was developed and found to detect MERS‐CoV‐specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS‐CoV S protein. Neither ELISA system exhibited significant intra‐assay or inter‐assay variation, indicating good reproducibility. Moreover, the inter‐day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS‐CoV.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0385-5600eISSN: 1348-0421DOI: 10.1111/1348-0421.12643Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7168444
- Format
- –
- Schlagworte
- Animals, Antibodies, Antibodies, Monoclonal, Antibodies, Viral - blood, Antibodies, Viral - immunology, Antibodies, Viral - isolation & purification, Antigens, Antigens, Viral - immunology, Antigens, Viral - isolation & purification, Baculoviridae - genetics, Coronaviridae, Coronavirus Infections - diagnosis, Coronavirus Infections - immunology, Coronavirus Infections - virology, Coronaviruses, Dipeptidyl-peptidase IV, ELISA, Enzyme-linked immunosorbent assay, Enzyme-Linked Immunosorbent Assay - methods, Escherichia coli - genetics, Gene Expression, Humans, Immunoglobulins, Immunologic Tests - methods, Middle East respiratory syndrome coronavirus, Middle East Respiratory Syndrome Coronavirus - genetics, Middle East Respiratory Syndrome Coronavirus - immunology, Original, Outbreaks, Patients, Peptidase, Protein Binding - genetics, Protein Binding - immunology, Protein Interaction Domains and Motifs, Proteins, Rats, Rats, Wistar, receptor binding domain, Receptors, Virus - immunology, Recombinant Proteins - genetics, Recombinant Proteins - immunology, Reproducibility of Results, Respiratory diseases, RNA viruses, Sensitivity and Specificity, Sf9 Cells, Spike Glycoprotein, Coronavirus - genetics, Spike Glycoprotein, Coronavirus - immunology, spike protein, Vaccines, Viral Matrix Proteins - genetics, Viral Matrix Proteins - immunology, Viruses