Details

Autor(en) / Beteiligte

• Chu, Shirley
• Wylie, Todd N.
• Wylie, Kristine M.
• Johnson, Gayle C.
• Skidmore, Zachary L.
• Fleer, Maren
• Griffith, Obi L.
• Bryan, Jeffrey N.

Titel

A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors

Ist Teil von

• Veterinary microbiology, 2020-01, Vol.240, p.108491-108491, Article 108491

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •A comprehensive high-throughput viral sequencing strategy (ViroCap) was adapted for use with feline tumors.•Papillomavirus was not commonly associated with feline oral squamous cell carcinoma.•Feline oral squamous cell carcinoma is a good model for HPV-negative head and neck squamous cell carcinoma in people.•The virome of FOSCC and normal feline oral mucosa included feline foamy virus, torque teno virus, herpes and papillomavirus, FIV and EBV.•Co-occurrence of Epstein Barr Virus and feline papillomavirus-3 was found found in a feline oral squamous cell carcinoma sample. Feline oral squamous cell carcinoma (FOSCC) may be the best naturally-occurring model of human head and neck squamous cell carcinoma (HNSCC). HNSCC can be broadly divided into human papillomavirus (HPV)-negative cancers and HPV-positive cancers where HPV is the causative agent. Previous studies in FOSCC have used both species-specific and species-nonspecific PCR primers that may be insensitive to the detection of PVs and other viruses that may be divergent from known sequences. ViroCap is a targeted capture and next generation sequencing tool that was designed to identify all known vertebrate DNA and RNA viruses. In this study we used a metagenomic approach using ViroCap for DNA viruses in 20 FOSCC, 9 normal feline oral mucosal, and 8 suspected PV positive control samples. We tested the hypothesis that viruses would be enriched in FOSCC compared to normal oral mucosa. The virome of the FOSCC and normal feline oral mucosa consisted of feline foamy virus in 7/20 and 2/9 (35% and 22%), feline torque teno virus in 2/20 and 0/9 (10% and 0%), alphaherpesvirus in 2/10 and 0/9 (10% and 0%), FIV (0% and 22%), Epstein-Barr virus in 1/20 and 0/9 (5% and 0%) and feline papillomavirus in 1/20 and 0/9 samples (5% and 0% respectively). Felis catus papillomavirus-3 was found in 1 of 20 FOSCC samples. A virus was not associated consistently with FOSCC. If PVs have a role in FOSCC it is at most a supplementary or uncommon role. FOSCC appears most closely related to HPV-negative HNSCC. Future research on FOSCC should focus on identifying genetic and environmental causes.