Details

Autor(en) / Beteiligte

• Lu, Shuai
• Chen, Yingzhu
• Qin, Kun
• Zhou, Jianfang
• Lou, Yongliang
• Tan, Wenjie

Titel

Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China

Ist Teil von

• Science China. Life sciences, 2016-06, Vol.59 (6), p.615-621

Ort / Verlag

Beijing: Science China Press

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• To characterize the antigenicity of nucleocapsid proteins（NP） derived from canine coronavirus（CCo V） and canine respiratory coronavirus（CRCo V） in China, the N genes of CCo V（CCo V-BJ70） and CRCo V（CRCo V-BJ202） were cloned from swabs obtained from diseased pet dogs in Beijing and then sequenced. The recombinant NPs（r NPs） were expressed in Escherichia coli and purified by nickel-affinity column and size exclusion chromatography. Sequencing data indicated that the N genes of CCo V-BJ70 and CRCo V-BJ202 belonging to two distinctly different groups were relatively conserved within each subgroup. Sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） results showed that r NPs of CCo V and CRCo V were expressed efficiently and isolated with a final purity of over 95%. Western blot analysis revealed the r NP from CRCo V could cross-react with mice antisera against human coronavirus（HCo V-229 E, NL63, OC43, HKU1）, while r NP of CCo V had cross-reactivity with only anti-sera against viruses belonging to the same group（HCo V-229 E and NL63）. In summary, CCo V and CRCo V r NPs were successfully expressed in E. coli and showed antigenic cross-reactivity with antisera raised against human coronaviruses. These findings indicate that further serologic studies on coronavirus infections at the animal-human interface are needed.