Details

Autor(en) / Beteiligte

• Hau, Hoi Ting A
• Ogundele, Oluwaseun
• Hibbert, Andrew H
• Monfries, Clinton A L
• Exelby, Katherine
• Wood, Natalie J
• Nevarez-Mejia, Jessica
• Carbajal, M Alejandra
• Fleck, Roland A
• Dermit, Maria
• Mardakheh, Faraz K
• Williams-Ward, Victoria C
• Pipalia, Tapan G
• Conte, Maria R
• Hughes, Simon M

Titel

Maternal Larp6 controls oocyte development, chorion formation and elevation

Ist Teil von

• Development (Cambridge), 2020-02, Vol.147 (4)

Ort / Verlag

England: The Company of Biologists Ltd

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• La-related protein 6 (Larp6) is a conserved RNA-binding protein found across eukaryotes that has been suggested to regulate collagen biogenesis, muscle development, ciliogenesis, and various aspects of cell proliferation and migration. Zebrafish have two Larp6 family genes: and Viable and fertile single and double homozygous and zygotic mutants revealed no defects in muscle structure, and were indistinguishable from heterozygous or wild-type siblings. However, mutant females produced eggs with chorions that failed to elevate fully and were fragile. Eggs from single mutant females showed minor chorion defects, but chorions from eggs laid by double mutant females were more defective than those from single mutants. Electron microscopy revealed defective chorionogenesis during oocyte development. Despite this, maternal zygotic single and double mutants were viable and fertile. Mass spectrometry analysis provided a description of chorion protein composition and revealed significant reductions in a subset of zona pellucida and lectin-type proteins between wild-type and mutant chorions that paralleled the severity of the phenotype. We conclude that Larp6 proteins are required for normal oocyte development, chorion formation and egg activation.