Details

Autor(en) / Beteiligte

• Glowacki, Robert W.P.
• Pudlo, Nicholas A.
• Tuncil, Yunus
• Luis, Ana S.
• Sajjakulnukit, Peter
• Terekhov, Anton I.
• Lyssiotis, Costas A.
• Hamaker, Bruce R.
• Martens, Eric C.

Titel

A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner

Ist Teil von

• Cell host & microbe, 2020-01, Vol.27 (1), p.79-92.e9

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to access ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. We used molecular genetics to probe the required functions of the system and the nature of the nutrient source(s) underlying this phenotype. Two RUS-encoded ribokinases were the only components required for this effect, presumably because they generate ribose-phosphate derivatives from products of an unlinked but essential nucleoside phosphorylase. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and the potential for unexpected dependencies among systems that mediate colonization and persistence. [Display omitted] •Human gut Bacteroidetes and their relatives have diverse ribose-scavenging systems•A B. thetaiotaomicron ribose-utilization system (RUS) is needed on a plant diet•RUS ribokinases are the critical diet-specific determinants•Ribokinases yield ribose-1,5-bisphosphate from cleaved product of an unlinked gene Glowacki et al. show that the ability of Bacteroides thetaiotaomicron to use ribose derived from nucleosides is an important function in vivo on a plant-fiber-rich diet. Ribokinases encoded in a ribose-utilization system (RUS) and an unlinked nucleoside phosphorylase mediate this effect in vivo through generation of ribose-1,5-bisphosphate.