Details

Autor(en) / Beteiligte

• Probst, Florian Andreas
• Fliefel, Riham
• Burian, Egon
• Probst, Monika
• Eddicks, Matthias
• Cornelsen, Matthias
• Riedl, Christina
• Seitz, Hermann
• Aszódi, Attila
• Schieker, Matthias
• Otto, Sven

Titel

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Ist Teil von

• Scientific reports, 2020-02, Vol.10 (1), p.2062-2062, Article 2062

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixteen skeletally mature miniature pigs were divided into two groups: Control group and scaffolds seeded with osteogenic differentiated pADSCs (n = 8/group). TCP-PLGA scaffolds with or without cells were integrated in the mandibular critical size defects and fixed by titanium osteosynthesis plates. After 12 weeks, ADSCs seeded scaffolds ( n  = 7) demonstrated significantly higher bone volume (34.8% ± 4.80%) than scaffolds implanted without cells ( n  = 6, 22.4% ± 9.85%) in the micro-CT ( p  < 0.05). Moreover, an increased amount of osteocalcin deposition was found in the test group in comparison to the control group (27.98 ± 2.81% vs 17.10 ± 3.57%, p < 0.001). In conclusion, ADSCs seeding on ceramic/polymer scaffolds improves bone regeneration in large mandibular defects. However, further improvement with regard to the osteogenic capacity is necessary to transfer this concept into clinical use.