International journal of molecular sciences, 2019-12, Vol.21 (1), p.49
2019
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- Autor(en) / Beteiligte
- Titel
- Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts
- Ist Teil von
- International journal of molecular sciences, 2019-12, Vol.21 (1), p.49
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was found that ADSC-CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-κB). In addition, ADSC-CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression of collagen synthesis enhancer gene transforming growth factor-β (TGF-β). The expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging activities of ADSC-CM in cell-based models.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21010049Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6981944
- Format
- –
- Schlagworte
- Activator protein 1, Adipose Tissue - cytology, Adipose Tissue - metabolism, Aging, Aging (artificial), Antioxidants, Apoptosis, Binding sites, Cell culture, Cell Culture Techniques, Cells, Cultured, Collagen, Culture Media, Conditioned - pharmacology, Cytokines, Cytokines - metabolism, Fibroblasts, Fibroblasts - cytology, Fibroblasts - drug effects, Fibroblasts - radiation effects, Gene Expression Regulation - drug effects, Gene Expression Regulation - radiation effects, Growth factors, Heme, Heme oxygenase (decyclizing), Humans, Interleukin 6, Interstitial collagenase, Keratinocytes, Keratinocytes - cytology, Keratinocytes - drug effects, Keratinocytes - radiation effects, Kinases, Matrix metalloproteinase, Matrix Metalloproteinase 1 - metabolism, Matrix metalloproteinases, Mesenchymal stem cells, Mesenchymal Stem Cells - cytology, Mesenchymal Stem Cells - metabolism, Metalloproteinase, NF-κB protein, Organs, Oxygenase, Phosphorylation, Procollagen, Proteins, Radiation, Signal transduction, Signal Transduction - drug effects, Signal Transduction - radiation effects, Skin Aging - drug effects, Stem cells, Transcription factors, Transforming growth factor-b, Ultraviolet Rays - adverse effects, Wound healing