Details

Autor(en) / Beteiligte

• Melamed, Sahar
• Adams, Philip P.
• Zhang, Aixia
• Zhang, Hongen
• Storz, Gisela

Titel

RNA-RNA Interactomes of ProQ and Hfq Reveal Overlapping and Competing Roles

Ist Teil von

• Molecular cell, 2020-01, Vol.77 (2), p.411-425.e7

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Base pairing RNAs modulate gene expression in all studied organisms. In many bacteria, the base pairing between most small regulatory RNAs (sRNAs) and their targets is mediated by the Hfq RNA chaperone. However, recent studies have shown FinO-domain proteins also bind sRNAs. To examine the global contribution of the FinO-domain ProQ protein in Escherichia coli, we carried out RIL-seq to identify RNA pairs bound to this protein. The RNA-RNA interactome for ProQ contains hundreds of pairs. Intriguingly, a significant fraction of the ProQ-bound RNA pairs are also found associated with Hfq, indicating overlapping, complementary, or competing roles for the two proteins. Characterization of one novel RNA pair bound by both chaperones revealed that while Hfq is required for RNA sponge-mediated downregulation of the sRNA, ProQ can inhibit this regulation. Overall, our results uncover increased complexity in RNA regulatory networks involving RNA chaperone proteins, RNases, sRNAs, and mRNAs. [Display omitted] •RNA-RNA interactomes for RNA chaperones ProQ and Hfq are identified by RIL-seq•A significant fraction of the RNA-RNA pairs on ProQ also are found on Hfq•sRNA-mediated regulation is impacted by growth conditions•ProQ blocks RNase III- and Hfq-mediated downregulation by a novel sRNA sponge Melamed and Adams et al. compare the RNA-RNA interactomes of the well-characterized Hfq and the understudied ProQ RNA chaperones. They examine overlapping RNA pairs bound by both proteins, identify a new RNA sponge for the RybB small RNA, and document that ProQ can block Hfq activity.