Details

Autor(en) / Beteiligte

• Girardin, Ariane
• Wang, Tongming
• Ding, Yi
• Keller, Jean
• Buendia, Luis
• Gaston, Mégane
• Ribeyre, Camille
• Gasciolli, Virginie
• Auriac, Marie-Christine
• Vernié, Tatiana
• Bendahmane, Abdelhafid
• Ried, Martina Katharina
• Parniske, Martin
• Morel, Patrice
• Vandenbussche, Michiel
• Schorderet, Martine
• Reinhardt, Didier
• Delaux, Pierre-Marc
• Bono, Jean-Jacques
• Lefebvre, Benoit

Titel

LCO Receptors Involved in Arbuscular Mycorrhiza Are Functional for Rhizobia Perception in Legumes

Ist Teil von

• Current biology, 2019-12, Vol.29 (24), p.4249-4259.e5

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM receptor-like kinases LYK10 are impaired in AM formation. Petunia and tomato LYK10 proteins have a high affinity for LCOs (Kd in the nM range) comparable to that previously reported for a legume LCO receptor essential for the RNS. Interestingly, the tomato and petunia LYK10 promoters, when introduced into a legume, were active in nodules similarly to the promoter of the legume orthologous gene. Moreover, tomato and petunia LYK10 coding sequences restored nodulation in legumes mutated in their orthologs. This combination of genetic and biochemical data clearly pinpoints Solanaceous LYK10 as part of an ancestral LCO perception system involved in AM establishment, which has been directly recruited during evolution of the RNS in legumes. •Mutants in Solanaceaous LysM receptors LYK10 are impaired in arbuscular mycorrhiza•LYK10 proteins have a high affinity for lipo-chitooligosaccharidic signal molecules•LYK10 promoter is expressed in arbuscule-containing cells in tomato roots•Solanaceaous LYK10 can restore nodulation in legumes mutated in their orthologs Soil rhizobial bacteria and arbuscular mycorrhizal (AM) fungi produce lipo-chitooligosaccharidic (LCO) signal molecules. Girardin et al. show that plant LCO receptors are involved in establishment of the ancient AM symbiosis and have been recruited during evolution for establishment of the nitrogen-fixing root nodule symbiosis with rhizobia.