Communications biology, 2019-12, Vol.2 (1), p.474, Article 474
2019
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- Autor(en) / Beteiligte
- Titel
- Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species
- Ist Teil von
- Communications biology, 2019-12, Vol.2 (1), p.474, Article 474
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι- car rageenan specific p olysaccharide utilization l ocus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche. Hettle et al. investigate the ability of marine Pseudoalteromonas sp. to metabolise carrageenan, a polysaccharide abundant in red algae. They isolate and characterise previously unstudied strains and find that the recently identified κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) is required for growth on carrageenan, and biochemically map out many of the steps.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2399-3642eISSN: 2399-3642DOI: 10.1038/s42003-019-0721-yTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6923384
- Format
- –
- Schlagworte
- 45/43, 45/91, 631/45/221, 631/45/535, 631/45/535/1266, 631/45/607, 631/45/72, 82, Algae, Aquatic Organisms - metabolism, BASIC BIOLOGICAL SCIENCES, Binding Sites, Biology, Biomedical and Life Sciences, Carbohydrates, Carrageenan - chemistry, Carrageenan - metabolism, Enzymes, Gene Order, Glycobiology, Glycoside Hydrolases - chemistry, Glycoside Hydrolases - metabolism, Life Sciences, Metabolic Networks and Pathways, Metabolic pathways, Metabolism, Models, Molecular, Niches, Open Reading Frames, Polysaccharides, Protein Binding, Pseudoalteromonas, Pseudoalteromonas - genetics, Pseudoalteromonas - metabolism, Seaweeds, Structural biology, Structure-Activity Relationship, Structure-function relationships, X-ray crystallography