The Plant journal : for cell and molecular biology, 2019-12, Vol.100 (5), p.879-891
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Biosynthesis of the anti‐diabetic metabolite montbretin A: glucosylation of the central intermediate mini‐MbA
- Ist Teil von
- The Plant journal : for cell and molecular biology, 2019-12, Vol.100 (5), p.879-891
- Ort / Verlag
- England: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary Type 2 diabetes (T2D) affects over 320 million people worldwide. Healthy lifestyles, improved drugs and effective nutraceuticals are different components of a response against the growing T2D epidemic. The specialized metabolite montbretin A (MbA) is being developed for treatment of T2D and obesity due to its unique pharmacological activity as a highly effective and selective inhibitor of the human pancreatic α‐amylase. MbA is an acylated flavonol glycoside found in small amounts in montbretia (Crocosmia × crocosmiiflora) corms. MbA cannot be obtained in sufficient quantities for drug development from its natural source or by chemical synthesis. To overcome these limitations through metabolic engineering, we are investigating the genes and enzymes of MbA biosynthesis. We previously reported the first three steps of MbA biosynthesis from myricetin to myricetin 3‐O‐(6′‐O‐caffeoyl)‐glucosyl rhamnoside (mini‐MbA). Here, we describe the sequence of reactions from mini‐MbA to MbA, and the discovery and characterization of the gene and enzyme responsible for the glucosylation of mini‐MbA. The UDP‐dependent glucosyltransferase CcUGT3 (UGT703E1) catalyzes the 1,2‐glucosylation of mini‐MbA to produce myricetin 3‐O‐(glucosyl‐6′‐O‐caffeoyl)‐glucosyl rhamnoside. Co‐expression of CcUGT3 with genes for myricetin and mini‐MbA biosynthesis in Nicotiana benthamiana validated its biological function and expanded the set of genes available for metabolic engineering of MbA. Significance Statement Montbretin A (MbA) is a complex specialized metabolite found in the ornamental plant montbretia and being developed as a treatment for type 2 diabetes. Knowledge of MbA biosynthesis, including a central UDP‐dependent glucosyltransferase reaction described here, is essential to enable synthetic biology of MbA production via metabolic engineering.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-7412eISSN: 1365-313XDOI: 10.1111/tpj.14493Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6899944
- Format
- –
- Schlagworte
- bioproduct, Biosynthesis, Caffeic Acids - chemistry, Caffeic Acids - metabolism, Chemical synthesis, Corms, Crocosmia × crocosmiiflora, Diabetes mellitus (non-insulin dependent), Diabetes Mellitus, Type 2 - drug therapy, Drug development, Epidemics, Flavones - biosynthesis, Flavones - chemistry, Flavones - pharmacology, Flavones - therapeutic use, flavonoid, Flavonoids - chemistry, Flavonoids - metabolism, Flavonols - chemistry, Flavonols - metabolism, Functional foods & nutraceuticals, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Plant - genetics, Genes, Glucose - chemistry, Glucose - metabolism, Glucosyltransferase, Glucosyltransferases - metabolism, Glycosides - chemistry, Glycosides - metabolism, Glycosylation, Hypoglycemic Agents - chemistry, Hypoglycemic Agents - metabolism, Hypoglycemic Agents - pharmacology, Hypoglycemic Agents - therapeutic use, Iridaceae - chemistry, Iridaceae - enzymology, Metabolic engineering, Metabolic Engineering - methods, Metabolism, Metabolites, Nicotiana - metabolism, Organic chemistry, Original, Pancreas, Phylogeny, Plant Proteins - metabolism, plant secondary metabolism, plant specialized metabolism, Plant Stems - chemistry, Plant Stems - metabolism, plants and human health, Plants, Genetically Modified, Rhamnose - chemistry, Rhamnose - metabolism, Secondary Metabolism, synthetic biology, Synthetic Biology - methods, Transcriptome - genetics, Trisaccharides - biosynthesis, Trisaccharides - chemistry, Trisaccharides - pharmacology, Trisaccharides - therapeutic use, type 2 diabetes, UDP‐dependent glycosyltransferase, Xylose - chemistry, Xylose - metabolism, α-Amylase