Details

Autor(en) / Beteiligte

• Davis, William J.
• Amses, Kevin R.
• Benny, Gerald L.
• Carter-House, Derreck
• Chang, Ying
• Grigoriev, Igor
• Smith, Matthew E.
• Spatafora, Joseph W.
• Stajich, Jason E.
• James, Timothy Y.

Titel

Genome-scale phylogenetics reveals a monophyletic Zoopagales (Zoopagomycota, Fungi)

Ist Teil von

• Molecular phylogenetics and evolution, 2019-04, Vol.133 (C), p.152-163

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •Produced a phylogenetic analysis of uncultured predacious fungi using genomes generated from single cell genomic methods.•Monophyletic Zoopagales.•Ancestral state reconstructions could not resolve the trophic state of the zoopagalean ancestor. Previous genome-scale phylogenetic analyses of Fungi have under sampled taxa from Zoopagales; this order contains many predacious or parasitic genera, and most have never been grown in pure culture. We sequenced the genomes of 4 zoopagalean taxa that are predators of amoebae, nematodes, or rotifers and the genome of one taxon that is a parasite of amoebae using single cell sequencing methods with whole genome amplification. Each genome was a metagenome, which was assembled and binned using multiple techniques to identify the target genomes. We inferred phylogenies with both super matrix and coalescent approaches using 192 conserved proteins mined from the target genomes and performed ancestral state reconstructions to determine the ancestral trophic lifestyle of the clade. Our results indicate that Zoopagales is monophyletic. Ancestral state reconstructions provide moderate support for mycoparasitism being the ancestral state of the clade.