Proceedings of the National Academy of Sciences - PNAS, 2019-11, Vol.116 (45), p.22485-22490
2019
Details
- Autor(en) / Beteiligte
- Titel
- Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2019-11, Vol.116 (45), p.22485-22490
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Self-assembly is widely used by biological systems to build functional nanostructures, such as the protein capsids of RNA viruses. But because assembly is a collective phenomenon involving many weakly interacting subunits and a broad range of timescales, measurements of the assembly pathways have been elusive. We use interferometric scattering microscopy to measure the assembly kinetics of individual MS2 bacteriophage capsids around MS2 RNA. By recording how many coat proteins bind to each of many individual RNA strands, we find that assembly proceeds by nucleation followed by monotonic growth. Our measurements reveal the assembly pathways in quantitative detail and also show their failure modes. We use these results to critically examine models of the assembly process.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1909223116Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6842639
- Format
- –
- Schlagworte
- Biological Sciences, Capsid - chemistry, Capsid - metabolism, Capsid Proteins - chemistry, Capsid Proteins - genetics, Capsid Proteins - metabolism, Capsids, Failure modes, Genome, Viral, Genomes, Kinetics, Levivirus - chemistry, Levivirus - genetics, Levivirus - growth & development, Levivirus - physiology, Nucleation, Phages, Physical Sciences, Proteins, Recording, RNA phages, RNA viruses, RNA Viruses - chemistry, RNA Viruses - genetics, RNA Viruses - growth & development, RNA Viruses - physiology, RNA, Viral - chemistry, RNA, Viral - genetics, RNA, Viral - metabolism, Self-assembly, Virion - chemistry, Virion - genetics, Virion - physiology, Virus Assembly, Viruses