Details

Autor(en) / Beteiligte

• Dorshow, Richard B.
• Johnson, J. R.
• Debreczeny, Martin P.
• Riley, I. Rochelle
• Shieh, Jeng-Jong
• Rogers, Thomas E.
• Hall-Moore, Carla
• Shaikh, Nurmohammad
• Rouggly-Nickless, L. Colleen
• Tarr, Phillip I.

Titel

Transdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement

Ist Teil von

• Biomedical optics express, 2019-10, Vol.10 (10), p.5103-5116

Ort / Verlag

Optical Society of America

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The intestinal mucosal barrier prevents macromolecules and pathogens from entering the circulatory stream. Tight junctions in this barrier are compromised in inflammatory bowel diseases, environmental enteropathy, and enteric dysfunction. Dual sugar absorption tests are a standard method for measuring gastrointestinal integrity, however, these are not clinically amenable. Herein, we report on a dual fluorophore system and fluorescence detection instrumentation for which gastrointestinal permeability is determined in a rat small bowel disease model from the longitudinal measured transdermal fluorescence of each fluorophore. This fluorophore technology enables a specimen-free, noninvasive, point-of-care gastrointestinal permeability measurement which should be translatable to human clinical studies.