Details

Autor(en) / Beteiligte

• Noriega, Daniel D
• Arias, Paula L
• Barbosa, Helena R
• Arraes, Fabricio B M
• Ossa, Gustavo A
• Villegas, Bernardo
• Coelho, Roberta R
• Albuquerque, Erika V S
• Togawa, Roberto C
• Grynberg, Priscila
• Wang, Haichuan
• Vélez, Ana M
• Arboleda, Jorge W
• Grossi-de-Sa, Maria F
• Silva, Maria C M
• Valencia-Jiménez, Arnubio

Titel

Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei

Ist Teil von

• Scientific reports, 2019-09, Vol.9 (1), p.12804-13, Article 12804

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Coffee production is a global industry valued at approximately 173 billion US dollars. One of the main challenges facing coffee production is the management of the coffee berry borer (CBB), Hypothenemus hampei, which is considered the primary arthropod pest of coffee worldwide. Current control strategies are inefficient for CBB management. Although biotechnological alternatives, including RNA interference (RNAi), have been proposed in recent years to control insect pests, characterizing the genetics of the target pest is essential for the successful application of these emerging technologies. In this study, we employed RNA-seq to obtain the transcriptome of three developmental stages of the CBB (larva, female and male) to increase our understanding of the CBB life cycle in relation to molecular features. The CBB transcriptome was sequenced using Illumina Hiseq and assembled de novo. Differential gene expression analysis was performed across the developmental stages. The final assembly produced 29,434 unigenes, of which 4,664 transcripts were differentially expressed. Genes linked to crucial physiological functions, such as digestion and detoxification, were determined to be tightly regulated between the reproductive and nonreproductive stages of CBB. The data obtained in this study help to elucidate the critical roles that several genes play as regulatory elements in CBB development.