Details

Autor(en) / Beteiligte

• Van de Weyer, Anna-Lena
• Monteiro, Freddy
• Furzer, Oliver J.
• Nishimura, Marc T.
• Cevik, Volkan
• Witek, Kamil
• Jones, Jonathan D.G.
• Dangl, Jeffery L.
• Weigel, Detlef
• Bemm, Felix

Titel

A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana

Ist Teil von

• Cell, 2019-08, Vol.178 (5), p.1260-1272.e14

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consistent with extensive balancing and positive selection, NLRs are encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define a nearly complete species-wide pan-NLRome in Arabidopsis thaliana based on sequence enrichment and long-read sequencing. The pan-NLRome largely saturates with approximately 40 well-chosen wild strains, with half of the pan-NLRome being present in most accessions. We chart NLR architectural diversity, identify new architectures, and quantify selective forces that act on specific NLRs and NLR domains. Our study provides a blueprint for defining pan-NLRomes. [Display omitted] •Species-wide NLR diversity is high but not unlimited•A large fraction of NLR diversity is recovered with 40–50 accessions•Presence/absence variation in NLRs is widespread, resulting in a mosaic population•A high diversity of NLR-integrated domains favor known virulence targets In plants, NLR proteins are important intracellular receptors with roles in innate immunity and disease resistance. This work provides a panoramic view of this diverse and complicated gene family in the model species A. thaliana and provides a foundation for the identification and functional study of disease-resistance genes in agronomically important species with complex genomes.