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Details

Autor(en) / Beteiligte
Titel
Identification of anthocyanin biosynthesis genes in rice pericarp using PCAMP
Ist Teil von
  • Plant biotechnology journal, 2019-09, Vol.17 (9), p.1700-1702
Ort / Verlag
England: John Wiley & Sons, Inc
Erscheinungsjahr
2019
Quelle
Wiley Online Library Core Title
Beschreibungen/Notizen
  • [...]SHOREmap requires a much larger sample size; the NGM studies the genes belongs to the recessive homozygous mutant phenotype; MutMap mainly identifies the single gene‐controlled quality traits; QTL‐seq constructs only two pools showing extreme opposite trait values for a given phenotype in a segregating progeny and maps 1–2 major genes for target trait; GWAS is applicable to natural population with a large sample size and thus its cost is high, and it is also difficult to detect the rare mutations and minor effective genes. [...]we compared the SNP‐index between every two Pool‐seq to map the genomic candidate regions. For the genomic candidate regions with overlapping physical positions on the same chromosome, the intersection regions were selected as the final genomic candidate regions. [...]the regions showing a significant association with anthocyanin biosynthesis‐related genes in rice pericarp are shown in Figure c. Three genomic candidate regions were adjacent to or contained the cloned genes of anthocyanin biosynthesis (Figure c). The number of SNPs in the genomic region nearby Rd was greatly reduced (Figure e). [...]the false‐positive result may be resulted from a decrease in nucleotide polymorphism within this genomic region.

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