Details

Autor(en) / Beteiligte

• Waks, Adrienne G
• Stover, Daniel G
• Guerriero, Jennifer L
• Dillon, Deborah
• Barry, William T
• Gjini, Evisa
• Hartl, Christina
• Lo, Wesley
• Savoie, Jennifer
• Brock, Jane
• Wesolowski, Robert
• Li, Zaibo
• Damicis, Adrienne
• Philips, Anne V
• Wu, Yun
• Yang, Fei
• Sullivan, Amy
• Danaher, Patrick
• Brauer, Heather Ann
• Osmani, Wafa
• Lipschitz, Mikel
• Hoadley, Katherine A
• Goldberg, Michael
• Perou, Charles M
• Rodig, Scott
• Winer, Eric P
• Krop, Ian E
• Mittendorf, Elizabeth A
• Tolaney, Sara M

Titel

The Immune Microenvironment in Hormone Receptor-Positive Breast Cancer Before and After Preoperative Chemotherapy

Ist Teil von

• Clinical cancer research, 2019-08, Vol.25 (15), p.4644-4655

Ort / Verlag

United States

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Hormone receptor-positive/HER2-negative (HR /HER2 ) breast cancer is associated with low levels of stromal tumor-infiltrating lymphocytes (sTIL) and PD-L1, and demonstrates poor responses to checkpoint inhibitor therapy. Evaluating the effect of standard chemotherapy on the immune microenvironment may suggest new opportunities for immunotherapy-based approaches to treating HR /HER2 breast tumors. HR /HER2 breast tumors were analyzed before and after neoadjuvant chemotherapy. sTIL were assessed histologically; CD8 cells, CD68 cells, and PD-L1 staining were assessed immunohistochemically; whole transcriptome sequencing and panel RNA expression analysis (NanoString) were performed. Ninety-six patients were analyzed from two cohorts ( = 55, Dana-Farber cohort; = 41, MD Anderson cohort). sTIL, CD8, and PD-L1 on tumor cells were higher in tumors with basal PAM50 intrinsic subtype. Higher levels of tissue-based lymphocyte (sTIL, CD8, PD-L1) and macrophage (CD68) markers, as well as gene expression markers of lymphocyte or macrophage phenotypes (NanoString or CIBERSORT), correlated with favorable response to neoadjuvant chemotherapy, but not with improved distant metastasis-free survival in these cohorts or a large gene expression dataset ( = 302). In paired pre-/postchemotherapy samples, sTIL and CD8 cells were significantly decreased after treatment, whereas expression analyses (NanoString) demonstrated significant increase of multiple myeloid signatures. Single gene expression implicated increased expression of immunosuppressive (M2-like) macrophage-specific genes after chemotherapy. The immune microenvironment of HR /HER2 tumors differs according to tumor biology. This cohort of paired pre-/postchemotherapy samples suggests a critical role for immunosuppressive macrophage expansion in residual disease. The role of macrophages in chemoresistance should be explored, and further evaluation of macrophage-targeting therapy is warranted.