Details

Autor(en) / Beteiligte

• Marchitto, Mark C.
• Dillen, Carly A.
• Liu, Haiyun
• Miller, Robert J.
• Archer, Nathan K.
• Ortines, Roger V.
• Alphonse, Martin P.
• Marusina, Alina I.
• Merleev, Alexander A.
• Wang, Yu
• Pinsker, Bret L.
• Byrd, Angel S.
• Brown, Isabelle D.
• Ravipati, Advaitaa
• Zhang, Emily
• Cai, Shuting S.
• Limjunyawong, Nathachit
• Dong, Xinzhong
• Yeaman, Michael R.
• Simon, Scott I.
• Shen, Wei
• Durum, Scott K.
• O’Brien, Rebecca L.
• Maverakis, Emanual
• Miller, Lloyd S.

Titel

Clonal Vγ6⁺Vδ4⁺ T cells promote IL-17–mediated immunity against Staphylococcus aureus skin infection

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2019-05, Vol.116 (22), p.10917-10926

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2019

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• T cell cytokines contribute to immunity against Staphylococcus aureus, but the predominant T cell subsets involved are unclear. In an S. aureus skin infection mouse model, we found that the IL- 17 response was mediated by γδ T cells, which trafficked from lymph nodes to the infected skin to induce neutrophil recruitment, proinflammatory cytokines IL-1α, IL-1β, and TNF, and host defense peptides. RNA-seq for TRG and TRD sequences in lymph nodes and skin revealed a single clonotypic expansion of the encoded complementarity-determining region 3 amino acid sequence, which could be generated by canonical nucleotide sequences of TRGV5 or TRGV6 and TRDV4. However, only TRGV6 and TRDV4 but not TRGV5 sequences expanded. Finally, Vγ6⁺ T cells were a predominant γδ T cell subset that produced IL-17A as well as IL-22, TNF, and IFNγ, indicating a broad and substantial role for clonal Vγ6⁺Vδ4⁺ T cells in immunity against S. aureus skin infections.