Details

Autor(en) / Beteiligte

• Rutkai, Ibolya
• Merdzo, Ivan
• Wunnava, Sanjay V
• Curtin, Genevieve T
• Katakam, Prasad VG
• Busija, David W

Titel

Cerebrovascular function and mitochondrial bioenergetics after ischemia-reperfusion in male rats

Ist Teil von

• Journal of cerebral blood flow and metabolism, 2019-06, Vol.39 (6), p.1056-1068

Ort / Verlag

London, England: SAGE Publications

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• The underlying factors promoting increased mitochondrial proteins, mtDNA, and dilation to mitochondrial-specific agents in male rats following tMCAO are not fully elucidated. Our goal was to determine the morphological and functional effects of ischemia/reperfusion (I/R) on mitochondria using electron microscopy, Western blot, mitochondrial oxygen consumption rate (OCR), and Ca2+ sparks activity measurements in middle cerebral arteries (MCAs) from male Sprague Dawley rats (Naïve, tMCAO, Sham). We found a greatly increased OCR in ipsilateral MCAs (IPSI) compared with contralateral (CONTRA), Sham, and Naïve MCAs. Consistent with our earlier findings, the expression of Mitofusin-2 and OPA-1 was significantly decreased in IPSI arteries compared with Sham and Naïve. Mitochondrial morphology was disrupted in vascular smooth muscle, but morphology with normal and perhaps greater numbers of mitochondria were observed in IPSI compared with CONTRA MCAs. Consistently, there were significantly fewer baseline Ca2+ events in IPSI MCAs compared with CONTRA, Sham, and Naïve. Mitochondrial depolarization significantly increased Ca2+ sparks activity in the IPSI, Sham, Naïve, but not in the CONTRA group. Our data indicate that altered mitochondrial structure and function occur in MCAs exposed to I/R and that these changes impact not only OCR but Ca2+ sparks activity in both IPSI and CONTRA MCAs.