Details

Autor(en) / Beteiligte

• Qin, Yuling
• Wu, Li
• Wang, Jingang
• Han, Rui
• Shen, Jingyu
• Wang, Jiasi
• Xu, Shihan
• Paguirigan, Amy L
• Smith, Jordan L
• Radich, Jerald P
• Chiu, Daniel T

Titel

A Fluorescence-Activated Single-Droplet Dispenser for High Accuracy Single-Droplet and Single-Cell Sorting and Dispensing

Ist Teil von

• Analytical chemistry (Washington), 2019-05, Vol.91 (10), p.6815-6819

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The ability to sort and dispense droplets accurately is essential to droplet-based single-cell analysis. Here, we describe a fluorescence-activated single-droplet dispenser (FASD) that is analogous to a conventional fluorescence-activated cell sorter, but sorts droplets containing single cells within an oil emulsion. The FASD system uses cytometric detection and electrohydrodynamic actuation-based single-droplet manipulation, allowing droplet isolation and dispensing with high efficiency and accuracy. The system is compatible with multiwell plates and can be integrated with existing microfluidic devices and large-scale screening systems. By enabling sorting based on single-cell reactions such as PCR, this platform will help expand the basis of cell sorting from mainly protein biomarkers to nucleic acid sequences and secreted biomolecules.