Details

Autor(en) / Beteiligte

• Turturice, Benjamin A.
• Gold, Diane R.
• Litonjua, Augusto A.
• Oken, Emily
• Rifas-Shiman, Sheryl
• Perkins, David L.
• Finn, Patricia W.

Titel

Lower perinatal exposure to Proteobacteria is an independent predictor of early childhood wheezing

Ist Teil von

• Journal of allergy and clinical immunology, 2019-01, Vol.143 (1), p.419-421.e5

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Proteobacteria are a diverse phyla of Gram-negative bacteria and, as compared with Bacteroidetes (also Gram-negative), LPS derived from Proteobacteria may have additional immunomodulatory properties.3 LPS in mattress dust has previously been negatively correlated with peripheral blood mononuclear cell IL-13 responses to allergen challenge in childhood.4 In concordance, administration of an LPS mimetic in murine models of asthma skews immune response toward IFN-γ (TH1 cytokine) production and suppresses airway hyperreactivity.5 Together, these data suggest that LPS exposure, both endogenous and exogenous, could suppress allergic responses implicated in the pathogenesis of childhood asthma. [...]we observed that lower circulating Proteobacteria DNA in umbilical cord blood serum is associated with maternal atopy, greater hazard of wheezing, and greater bronchodilator response within the first 7 years after birth. For testing associations between demographic data and cfbDNA composition, samples were normalized using median sum-scaling,E11 pairwise sample distances were calculated using the weighted UNIFRAC method,E9 ordinated using principal-component analysis, and tested for associations (Table E2) using the vegan envfit function.E12 Maternal atopy was assessed by interview response to “Has a health professional ever told you that you have asthma, hay fever, or eczema?” Phylum-level abundances were calculated by combining normalized reads of each OTU assigned to each phylum. Description of variable % (N) Maternal characteristics Atopy No 63.0 (17) Yes 33.3 (9) No response 3.7 (1) BMI (kg/m2), mean ± SD 24.9 ± 5.8 Race/ethnicity White 63.0 (17) Black 7.4 (2) Hispanic 7.4 (2) Other 18.5 (5) No response 3.7 (1) Smoking Never 85.2 (23) Former 7.4 (2) During pregnancy 7.4 (2) No response 0.0 (0) Antibiotics during pregnancy No 63.0 (17) Yes 33.3 (9) No response 3.7 (1) C-Section No 74.1 (20) Yes 25.9 (7) No response 0.0 (0) Child's characteristics Sex Male 55.6 (15) Female 44.4 (12) Race/ethnicity White 59.3 (16) Black 7.4 (2) Hispanic 7.4 (2) Other 22.2 (6) No response 0.0 (0) Gestational age (wk), mean ± SD 39.7 ± 1.2 Birth weight for gestational age z score, mean ± SD 0.41 ± 0.87∗ Table E1 Subjects' demographic characteristics Description of variable R2 P value Maternal characteristics Atopy 0.17 .07 BMI (kg/m2) 0.01 .95 Race/ethnicity 0.19 .28 Smoking 0.05 .70 Antibiotics during pregnancy 0.00 .90 C-Section 0.07 .15 Child's characteristics Sex 0.03 .41 Race/ethnicity 0.13 .54 Gestational age (wk) 0.05 .53 Birth weight for gestational age z score 0.15 .15 Table E2 Demographic associations with cfbDNA composition β SE P value 95% CI R∗ Imputed Univariate 1.283 0.383 .001 0.533 to 2.032 0.433 Adjusted† 1.030 0.331 .002 0.381 to 1.679 0.511 No missing data‡ Univariate 1.466 0.956 .125 −0.407 to 3.339 Adjusted† 1.382 1.058 .191 −0.692 to 3.496 Imputed wheeze§ Univariate 0.910 ]0.496 .066 −0.061 to 1.881 Adjusted† 0.793 0.523 .129 −0.230 to 1.815 Imputed nonwheeze‖ Univariate 1.619 0.625 .010 0.395 to 2.843 Adjusted† 1.309 0.662 .048 0.011 to 2.607 Table E3 Associated risk for low Proteobacteria cfbDNA exposure and time to first parental-reported wheezing event Year % (N) < Median % (N) > Median P value* First year 20.0 (2) 18.2 (2) .999 Second year 10.0 (1) 18.2 (2) .999 Third year 10.0 (1) 18.2 (2) .999 Fourth year 10.0 (1) 0.0 (0) .476 Fifth year 30.0 (3) 45.5 (5) .659 Sixth year 30.0 (3) 36.4 (4) .999 Seventh year 10.0 (1) 18.2 (2) .999 Total 17.1 (12) 22.1 (17) .536 Table E4 Missing data in each group per time point