Molecular cell, 2019-04, Vol.74 (1), p.132-142.e5
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry
- Ist Teil von
- Molecular cell, 2019-04, Vol.74 (1), p.132-142.e5
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Bacteria and archaea have evolved sophisticated adaptive immune systems that rely on CRISPR RNA (crRNA)-guided detection and nuclease-mediated elimination of invading nucleic acids. Here, we present the cryo-electron microscopy (cryo-EM) structure of the type I-F crRNA-guided surveillance complex (Csy complex) from Pseudomonas aeruginosa bound to a double-stranded DNA target. Comparison of this structure to previously determined structures of this complex reveals a ∼180-degree rotation of the C-terminal helical bundle on the “large” Cas8f subunit. We show that the double-stranded DNA (dsDNA)-induced conformational change in Cas8f exposes a Cas2/3 “nuclease recruitment helix” that is structurally homologous to a virally encoded anti-CRISPR protein (AcrIF3). Structural homology between Cas8f and AcrIF3 suggests that AcrIF3 is a mimic of the Cas8f nuclease recruitment helix. [Display omitted] •Structure of the type I-F CRISPR-RNA-guided surveillance complex bound to dsDNA•R-loop formation drives a conformational change that signals nuclease recruitment•Viral anti-CRISPR is a mimic of the C-terminal helical bundle of Cas8f The structure of a CRISPR-RNA-guided surveillance complex bound to dsDNA reveals a viral immune suppressor protein (AcrIF3) that mimics a critical subunit of the surveillance complex, which helps explain the mechanism of nuclease recruitment for degradation of foreign DNA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2019.02.001Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6521718
- Format
- –
- Schlagworte
- Acr, anti-CRISPR, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - immunology, Bacterial Proteins - metabolism, Cas, Cascade, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR, CRISPR-Associated Proteins - chemistry, CRISPR-Associated Proteins - genetics, CRISPR-Associated Proteins - immunology, CRISPR-Associated Proteins - metabolism, CRISPR-Cas Systems, crRNA, cryo-EM, Cryoelectron Microscopy, Csy, DNA, Bacterial - chemistry, DNA, Bacterial - genetics, DNA, Bacterial - metabolism, Models, Molecular, Molecular Mimicry, Nucleic Acid Conformation, Protein Conformation, Pseudomonas aeruginosa - enzymology, Pseudomonas aeruginosa - genetics, Pseudomonas aeruginosa - immunology, RNA, Bacterial - chemistry, RNA, Bacterial - genetics, RNA, Bacterial - metabolism, RNA, Guide, CRISPR-Cas Systems - chemistry, RNA, Guide, CRISPR-Cas Systems - genetics, RNA, Guide, CRISPR-Cas Systems - metabolism, Structure-Activity Relationship, type I-F, Viral Proteins - chemistry, Viral Proteins - genetics, Viral Proteins - immunology, Viral Proteins - metabolism