LASER THERAPY, 2018/12/28, Vol.27(4), pp.271-282
2018
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- Autor(en) / Beteiligte
- Titel
- Association of Bioglass/Collagen/Magnesium composites and low level irradiation: effects on bone healing in a model of tibial defect in rats
- Ist Teil von
- LASER THERAPY, 2018/12/28, Vol.27(4), pp.271-282
- Ort / Verlag
- Japan: International Phototherapy Association
- Erscheinungsjahr
- 2018
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- Background and Aims: Bioglass (BG) and Magnesium (Mg) composites have been used for bone tissueengineering proposes due to its osteogenic activity and increased mechanical properties respectively. Theintroduction of Collagen (Col) is a common and efficient approach for bone tissue engineering applicationstoward cell proliferation. Recently, studies demonstrated that BG/Col/Mg composites presentedproper mechanical properties and were non-cytotoxic. Although the osteogenic potential of BG/Col/Mgcomposites, in specific situations, biomaterials may not be capable of stimulating bone tissue. Therefore,combining biomaterial matrices and effective post-operative therapies (such as low level lasertherapy;LLLT) may be necessary to appropriately stimulate bone tissue. In this context, the aim of this study wasto develop intra- and extra-operatively bone regenerative therapeutical strategies, based on the associationof Col-enriched BG/Mg composites with LLLT. Materials and Methods: Thereby, an in vivo study, using tibial defect in Wistar rats, was performed in order to investigate the bone regenerative capacity. LLLT treatment (Ga-Al-As laser 808 nm, 30 mW, 2.8 J, 94 s) was performed 3 times a week, in non-consecutive days. Histology, histomorphometry, immunohistochemical analysis and mechanical test were done after 15 and 45 days post-implantation. Results: The results showed that Col could be successfully introduced into BG/Mg and the association of BG/Mg/Col and LLLT constituted an optimized treatment for accelerating material degradation and increasing bone deposition. Additionally, mechanical tests showed an increased maximal load for BG/Mg + LLLT compared to other groups. Conclusions: These results lead us to conclude that the Col enriched BG/Mg composites irradiated with LLLT presented superior biological and mechanical properties, demonstrating to be a promising bone graft.