Scientific reports, 2019-05, Vol.9 (1), p.7094, Article 7094
2019
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Differences in the molecular signatures of mucosal-associated invariant T cells and conventional T cells
- Ist Teil von
- Scientific reports, 2019-05, Vol.9 (1), p.7094, Article 7094
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mucosal-associated invariant T (MAIT) cells exhibit different characteristics from those of TCRα7.2 − conventional T cells. They play important roles in various inflammatory diseases, including rheumatoid arthritis and inflammatory bowel disease. MAIT cells express a single T cell receptor alpha chain, TCRα7.2 segment associated with Jα33 and CDR3 with fixed length, which recognizes bacteria-derived vitamin B metabolites. However, the characteristics of MAIT cells and TCRα7.2 + CD161 − T cells have never been compared. Here, we performed RNA sequencing to compare the properties of MAIT cells, TCRα7.2 − conventional T cells and TCRα7.2 + CD161 − T cells. Genome-wide transcriptomes of MAIT cells, TCRα7.2 − conventional T cells, and TCRα7.2 + CD161 − T cells were compared and analyzed using causal network analysis. This is the first report comparing the transcriptomes of MAIT cells, TCRα7.2 − conventional T cells and TCRα7.2 + CD161 − T cells. We also identified the predominant signaling pathways of MAIT cells, which differed from those of TCRα7.2 − conventional T cells and TCRα7.2 + CD161 − T cells, through a gene set enrichment test and upstream regulator analysis and identified the genes responsible for the characteristic MAIT cell phenotypes. Our study advances the complete understanding of MAIT biology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-019-43578-9Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6506535
- Format
- –
- Schlagworte
- 38, 38/39, 38/43, 45, 45/91, 631/114/2114, 631/250/248, 631/250/2502/248, 96, 96/31, Arthritis, Rheumatoid - immunology, Blood Donors, Complementarity-determining region 3, Gene Regulatory Networks, Genomes, Humanities and Social Sciences, Humans, Immunogenetics - methods, Inflammatory bowel diseases, Inflammatory diseases, Intestine, Killer Cells, Natural - metabolism, Lymphocytes, Lymphocytes T, Metabolites, Mucosa, Mucosal-Associated Invariant T Cells - immunology, multidisciplinary, NK Cell Lectin-Like Receptor Subfamily B - metabolism, Phenotype, Phenotypes, Receptors, Antigen, T-Cell, alpha-beta - metabolism, Rheumatoid arthritis, Ribonucleic acid, RNA, RNA-Seq - methods, Science, Science (multidisciplinary), T cell receptors, Th17 Cells - immunology, Th17 Cells - metabolism, Transcription Factors - genetics, Transcriptome